CIAE, Beijing, People’s Republic of China
Proton therapy has become one of the most important physiotherapies for tumors in the world, which can greatly improve the cure rate of tumors that are ineffective by conventional treatments. In addition, proton is also the main source of radiation in space environment. Therefore, it is of great scientific significance to use accelerators to carry out basic research on proton radiotherapy and space radiobiology, which can provide technical support and basic data for the optimal design of proton therapy and risk assessment of personnel in space environment. In this study, C57 mice were irradiated with 0, 0.2, 0.5 and 2 Gy by 90 MeV protons from 100 MeV cyclotron of China Institute of Atomic Energy. The mice were killed one day after irradiation. Body weight change and spleen organ coefficient were calculated. The expression of DNA damage-related protein γ H2AX was detected by western blotting. The results showed that compared with the control group, the body weight of mice in each irradiation group had no significant change, and the spleen organ coefficient decreased, indicating that the spleen atrophied after proton radiation, especially in 2 Gy. The results of Western blotting showed that the expression of γ H2AX in spleen increased significantly on the 1 day after irradiation, especially in 0.5 and 2 Gy, indicating that the spleen DNA damage was the serious on the 1 day after proton radiation.
