MOPA —  New Sciences from FELs   (22-Aug-11   15:30—17:30)

Paper	Title	Page
MOPA02	Development of a Microscopic Irradiation Technique for Delivering VIS-FELs to Single Cells through a Fine-tapered Glass Rod	16
	F. Shishikura Nihon University School of Medicine, Tokyo, Japan K. Hayakawa, Y. Hayakawa, M. Inagaki, K. Nakao, K. Nogami, T. Tanaka LEBRA, Funabashi, Japan
	The first lasing of LEBRA* succeeded in 2001 to produce near infrared FELs, by which the higher harmonics generated by means of the non-linear optical crystals now covered with visible and near infrared regions from 0.35 to 6 microns. The VIS-FELs are of particular interest and are expected to reveal photochemical reactions of single cells, even those in living organisms. To do this, it is a prerequisite to develop a micro-irradiation technique for targeting a single cell without photochemical effect to neighboring cells. We have established a microscopic irradiation technique with VIS-FELs through a fine glass rod. The FEL delivered through a fine-tapered glass rod with a diameter of about 5 microns has two major advantages compared with conventional microscope irradiation systems. The first is to deliver the FEL directly into targeted single cells in accordance with standard microinjection techniques used in developmental biology. The second is the ability to irradiate specific areas of the cytoplasm including cell organelle without severely damaging targeted cells. Using this technique, we also report micro-irradiation experiments on targeted single cells in living organisms. *Laboratory for Electron Beam Research and Application, Nihon University