FEL2011 - List of Authors (Nogami, K.)

Paper	Title	Page
MOPA02	Development of a Microscopic Irradiation Technique for Delivering VIS-FELs to Single Cells through a Fine-tapered Glass Rod	16
	F. Shishikura Nihon University School of Medicine, Tokyo, Japan K. Hayakawa, Y. Hayakawa, M. Inagaki, K. Nakao, K. Nogami, T. Tanaka LEBRA, Funabashi, Japan
	The first lasing of LEBRA* succeeded in 2001 to produce near infrared FELs, by which the higher harmonics generated by means of the non-linear optical crystals now covered with visible and near infrared regions from 0.35 to 6 microns. The VIS-FELs are of particular interest and are expected to reveal photochemical reactions of single cells, even those in living organisms. To do this, it is a prerequisite to develop a micro-irradiation technique for targeting a single cell without photochemical effect to neighboring cells. We have established a microscopic irradiation technique with VIS-FELs through a fine glass rod. The FEL delivered through a fine-tapered glass rod with a diameter of about 5 microns has two major advantages compared with conventional microscope irradiation systems. The first is to deliver the FEL directly into targeted single cells in accordance with standard microinjection techniques used in developmental biology. The second is the ability to irradiate specific areas of the cytoplasm including cell organelle without severely damaging targeted cells. Using this technique, we also report micro-irradiation experiments on targeted single cells in living organisms. *Laboratory for Electron Beam Research and Application, Nihon University

THPA28	Lasing of Near Infrared FEL with the Burst-mode Beam at LEBRA	535
	K. Nakao, K. Hayakawa, Y. Hayakawa, M. Inagaki, K. Nogami, T. Tanaka LEBRA, Funabashi, Japan
	Improvement of the electron beam injector system in the linac at the Laboratory for Electron Beam Research and Application (LEBRA) of Nihon University made possible to accelerate the burst-mode beam extracted from the conventional DC triode electron gun. The electron beam with the pulse width less than 1ns and the period of 44.8ns, which corresponds to the round-trip time in the FEL optical resonator, has been extracted by using a high-speed grid pulser (Kentec Inc.). Taking into account of the electron beam pulse width, sequence of two or three FEL pulses with the accelerating RF period was possible. In the lasing experiment a single FEL pulse or a row of two FEL pulses was observed using a streak camera. By the adjustment of the timing of the high-speed grid pulse generated in synchronous with the accelerating RF, lasing of a single FEL pulse in the single short beam pulse has been observed at an FEL wavelength of approximately 1800nm. The result suggests that a single FEL pulse with 44.8ns period is available in the wavelength range from 1600 to 6000nm at the LEBRA FEL system.

Paper

Title

Page

Development of a Microscopic Irradiation Technique for Delivering VIS-FELs to Single Cells through a Fine-tapered Glass Rod

16

F. Shishikura
Nihon University School of Medicine, Tokyo, Japan
K. Hayakawa, Y. Hayakawa, M. Inagaki, K. Nakao, K. Nogami, T. Tanaka
LEBRA, Funabashi, Japan

The first lasing of LEBRA* succeeded in 2001 to produce near infrared FELs, by which the higher harmonics generated by means of the non-linear optical crystals now covered with visible and near infrared regions from 0.35 to 6 microns. The VIS-FELs are of particular interest and are expected to reveal photochemical reactions of single cells, even those in living organisms. To do this, it is a prerequisite to develop a micro-irradiation technique for targeting a single cell without photochemical effect to neighboring cells. We have established a microscopic irradiation technique with VIS-FELs through a fine glass rod. The FEL delivered through a fine-tapered glass rod with a diameter of about 5 microns has two major advantages compared with conventional microscope irradiation systems. The first is to deliver the FEL directly into targeted single cells in accordance with standard microinjection techniques used in developmental biology. The second is the ability to irradiate specific areas of the cytoplasm including cell organelle without severely damaging targeted cells. Using this technique, we also report micro-irradiation experiments on targeted single cells in living organisms.
*Laboratory for Electron Beam Research and Application, Nihon University

THPA28

Lasing of Near Infrared FEL with the Burst-mode Beam at LEBRA

535

K. Nakao, K. Hayakawa, Y. Hayakawa, M. Inagaki, K. Nogami, T. Tanaka
LEBRA, Funabashi, Japan

Improvement of the electron beam injector system in the linac at the Laboratory for Electron Beam Research and Application (LEBRA) of Nihon University made possible to accelerate the burst-mode beam extracted from the conventional DC triode electron gun. The electron beam with the pulse width less than 1ns and the period of 44.8ns, which corresponds to the round-trip time in the FEL optical resonator, has been extracted by using a high-speed grid pulser (Kentec Inc.). Taking into account of the electron beam pulse width, sequence of two or three FEL pulses with the accelerating RF period was possible. In the lasing experiment a single FEL pulse or a row of two FEL pulses was observed using a streak camera. By the adjustment of the timing of the high-speed grid pulse generated in synchronous with the accelerating RF, lasing of a single FEL pulse in the single short beam pulse has been observed at an FEL wavelength of approximately 1800nm. The result suggests that a single FEL pulse with 44.8ns period is available in the wavelength range from 1600 to 6000nm at the LEBRA FEL system.