Author: Inagaki, M.
Paper Title Page
MOPA02 Development of a Microscopic Irradiation Technique for Delivering VIS-FELs to Single Cells through a Fine-tapered Glass Rod 16
 
  • F. Shishikura
    Nihon University School of Medicine, Tokyo, Japan
  • K. Hayakawa, Y. Hayakawa, M. Inagaki, K. Nakao, K. Nogami, T. Tanaka
    LEBRA, Funabashi, Japan
  
  The first las­ing of LEBRA* suc­ceeded in 2001 to pro­duce near in­frared FELs, by which the higher har­mon­ics gen­er­ated by means of the non-lin­ear op­ti­cal crys­tals now cov­ered with vis­i­ble and near in­frared re­gions from 0.35 to 6 mi­crons. The VIS-FELs are of par­tic­u­lar in­ter­est and are ex­pected to re­veal pho­to­chem­i­cal re­ac­tions of sin­gle cells, even those in liv­ing or­gan­isms. To do this, it is a pre­req­ui­site to de­velop a mi­cro-ir­ra­di­a­tion tech­nique for tar­get­ing a sin­gle cell with­out pho­to­chem­i­cal ef­fect to neigh­bor­ing cells. We have es­tab­lished a mi­cro­scopic ir­ra­di­a­tion tech­nique with VIS-FELs through a fine glass rod. The FEL de­liv­ered through a fine-ta­pered glass rod with a di­am­e­ter of about 5 mi­crons has two major ad­van­tages com­pared with con­ven­tional mi­cro­scope ir­ra­di­a­tion sys­tems. The first is to de­liver the FEL di­rectly into tar­geted sin­gle cells in ac­cor­dance with stan­dard mi­croin­jec­tion tech­niques used in de­vel­op­men­tal bi­ol­ogy. The sec­ond is the abil­ity to ir­ra­di­ate spe­cific areas of the cy­to­plasm in­clud­ing cell or­ganelle with­out se­verely dam­ag­ing tar­geted cells. Using this tech­nique, we also re­port mi­cro-ir­ra­di­a­tion ex­per­i­ments on tar­geted sin­gle cells in liv­ing or­gan­isms.
*Laboratory for Electron Beam Research and Application, Nihon University 		 
 
THPA28 Lasing of Near Infrared FEL with the Burst-mode Beam at LEBRA 535
 
  • K. Nakao, K. Hayakawa, Y. Hayakawa, M. Inagaki, K. Nogami, T. Tanaka
    LEBRA, Funabashi, Japan
  
  Im­prove­ment of the elec­tron beam in­jec­tor sys­tem in the linac at the Lab­o­ra­tory for Elec­tron Beam Re­search and Ap­pli­ca­tion (LEBRA) of Nihon Uni­ver­sity made pos­si­ble to ac­cel­er­ate the burst-mode beam ex­tracted from the con­ven­tional DC tri­ode elec­tron gun. The elec­tron beam with the pulse width less than 1ns and the pe­riod of 44.8ns, which cor­re­sponds to the round-trip time in the FEL op­ti­cal res­onator, has been ex­tracted by using a high-speed grid pulser (Ken­tec Inc.). Tak­ing into ac­count of the elec­tron beam pulse width, se­quence of two or three FEL pulses with the ac­cel­er­at­ing RF pe­riod was pos­si­ble. In the las­ing ex­per­i­ment a sin­gle FEL pulse or a row of two FEL pulses was ob­served using a streak cam­era. By the ad­just­ment of the tim­ing of the high-speed grid pulse gen­er­ated in syn­chro­nous with the ac­cel­er­at­ing RF, las­ing of a sin­gle FEL pulse in the sin­gle short beam pulse has been ob­served at an FEL wave­length of ap­prox­i­mately 1800nm. The re­sult sug­gests that a sin­gle FEL pulse with 44.8ns pe­riod is avail­able in the wave­length range from 1600 to 6000nm at the LEBRA FEL sys­tem.