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WEPSB14 |
Analysis of Single Biological Cells | |
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The direct analysis of trace metals in single cells and biological tissues is a challenging task that requires sophisticated analytical developments. The aim of this abstract is to present some recent achievements in the field of chemical element imaging of biological samples using ion beam micro-analysis. In the present work the potential of high-resolution RBS for detection of trace elements in an organic compound was tested. The main advantage of using RBS is that relative and absolute concentrations of present elements can be determined with very high accuracy. This study revealed the accumulation sites of micro- and macronutrients in seeds. Two selected examples of PIXE microanalysis in ecophysiology are presented. Studies of heavy metal distributions in seeds showed different filtration mechanisms of Zn/Pb and Fe/Mn, both enabling plants to cope with metals present in the environment.*
* A. Lagutin. Microscopy with Nanoscale Resolution Studied by High-Resolution RBS and Micro-PIXE. Proceedings of ICACS-26, 13-18 July 2014, abstract P73, P.124-125. |
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