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Kusnetsova, T. N.

Paper Title Page
THBAU03 FEL Irradiation Use for the Biochip Production Standardization  
 
  • T. N. Goryachkovskaya, T. N. Kusnetsova, V. A. Mordvinov, S. E. Peltek
    ICG SB RAS, Novosibirsk
  • A. S. Kozlov, S. B. Malyshkin, A. K. Petrov
    ICKC SB RAS, Novosibirsk
  • V. M. Popik, M. A. Scheglov
    BINP SB RAS, Novosibirsk
 
  The teraherz emission of the Budker INP Free electron laser was applied to the development of the scientific base for the biochip production standardization. The complementary pairings of hydrogen bonds of DNA nucleotides are the base of biochip applications. The technique is based on the method of soft nondestruction ablation developed by authors, which means the transfer of molecules to aerosol phase form solid substrate under action of the teraherz emission. The teraherz emission excites nonvalency of molecular bonds. Ablation of horseradish peroxidase biomoleculs was carried out and nondestruction of biomelecules was verified by using of an aerosol spectrometer and an electrophoresis in polyacrylamide gel. By hystochemical staining technique there was verified that peroxidase has retained its enzymatic activity. The molecular design of model biochip oligonucleotides and polymerase chain reaction analysis of ablation product were developed. The prototype of two different biochips on high resistance silicon substrate were manufactured. Requirements to the coating and immobilization of oligonucleotide probes on silicon substrate were developed. The first experiment on nondestruction ablation under teraherz emission of oligonucleotides from model biochip was achieved.  
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