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| MOPPH029 | Nondestructive Transfer of Complex Molecular Systems into Aerosol Phase by Means of Terahertz Irradiation of Free Electron Laser | |
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Original investigations of ablation of various substances under the action of submillimeter radiation of the free electron laser (FEL) developed and built at Budker Institute of Nuclear Physics were carried out at the Chemical and Biological user station of the Siberian Center for Photochemical Research. It was shown that ablation can be nondestructive. In order to determine the size distribution of aerosol products, we used the Diffusion Aerosol Spectrometer (DSA) developed at the ICKC SB RAS* and sampling for examination with electron microscope. The arrangement of experiments and equipment involved allowed us to determine the disperse composition of irradiation products within particle size range 3nm – 10mkm. At the present moment we have classified the aerosol products of ablation of the following substances: Crustal minerals, Fullerene-like complexes based on molybdenum; Polymers with different molecular masses, Biological macromolecules**. The most impressive results are obtained under ablation of biological macromolecules. In order to reveal whether the enzymatic activity of ablation products is conserved, we carried out a test for histochemical coloring of the collected aerosol of horse radish peroxidase. The test showed that this complicated enzyme remained active after ablation. We believe that this result is extremely important for biotechnology.
* A. N. Ankilov et al. Atmospheric Res., 2002, 62, 209-237.** A. K. Petrov et al. Nuclear Inst. and Methods in Physics Res., 2007, 575, 68-71. |
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| MOPPH047 | Influence of Terahertz Electromagnetic Radiation on Neurons In-vitro | |
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| Isolated neurons of mollusk in vitro at various stages of network neogenesis were studied earlier. The influence of submillimeter laser radiation (from 70 up to 418 mkm) it was investigated. 40-50 hours after laser irradiation (81.5 mkm) the formation of heterogeneities of the cell surface with the subsequent creation of arbitrarily directed process - like frames is observed in 12-15% of cells which were at the process shaping stage. In neurons which have formed processes, 15-20 hours after the exposition there is disturbance in the processes growth zones. The disturbance of formation of interneuron connections and thickening of processes terminations are observed. Similar alterations of cells are absent at smaller power density, exposition at other wavelengths, and in control neurons (without exposure). There is disturbance of cell adhesion to surface in 70-80% of neurons after irradiation at 418-mkm wavelength. Experiments on high power Novosibirsk teraherz free electron laser have shown influence of such radiation on functional characteristics alive neurons. The alterations observed can be the result of a modification of separate (possibly, individual) molecules of neuron regulation or structure-forming systems. | ||
| THBAU03 | FEL Irradiation Use for the Biochip Production Standardization | |
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| The teraherz emission of the Budker INP Free electron laser was applied to the development of the scientific base for the biochip production standardization. The complementary pairings of hydrogen bonds of DNA nucleotides are the base of biochip applications. The technique is based on the method of soft nondestruction ablation developed by authors, which means the transfer of molecules to aerosol phase form solid substrate under action of the teraherz emission. The teraherz emission excites nonvalency of molecular bonds. Ablation of horseradish peroxidase biomoleculs was carried out and nondestruction of biomelecules was verified by using of an aerosol spectrometer and an electrophoresis in polyacrylamide gel. By hystochemical staining technique there was verified that peroxidase has retained its enzymatic activity. The molecular design of model biochip oligonucleotides and polymerase chain reaction analysis of ablation product were developed. The prototype of two different biochips on high resistance silicon substrate were manufactured. Requirements to the coating and immobilization of oligonucleotide probes on silicon substrate were developed. The first experiment on nondestruction ablation under teraherz emission of oligonucleotides from model biochip was achieved. | ||
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