Author: Shishikura, F.
Paper Title Page
THPD06 Bio-luminescence/Scattering and its Dynamics in Enchytraeus Japonensis by Irradiation of Free Electron Laser 559
 
  • S. Kurumi, K. Suzuki, T. Taima
    Nihon University, Tokyo, Japan
  • K. Hayakawa, Y. Hayakawa, T. Tanaka
    LEBRA, Funabashi, Japan
  • F. Shishikura
    Nihon University School of Medicine, Tokyo, Japan
 
  We report on the experiment results of bioluminescence from Enchytraeus Japonensis by free electron laser (FEL) irradiation, and discuss about migration of bioluminescence on Enchytraeus Japonensis body. In this experiment, we observed behaviors or dynamics of FEL irradiated Enchytraeus japonensis for a few seconds by change coupled device (CCD) camera. One shot of FEL (wavelength: 2940 nm, pulse width: 200 fsec) which resonated with hydroxyl stretching vibration was irradiated to center of the Enchytraeus Japonensis head. After FEL irradiation, head was amputated, and orange bioluminescence was generated from around the laser irradiation area. Immediately after laser amputation of the head (0.1 seconds later), yellow bioluminescence was observed, and intensity of this luminescence was increasing after 0.3 seconds. This bioluminescence migrated into living cells from the head toward the trunk of the body. 10.0 seconds later, newly blue bioluminescence was generated from Enchytraeus Japonensis head. Wavelengths of these bioluminescence were as below, orange was from 600 nm to 650 nm, yellow was from 500 nm to 600 nm, blue was from 400 nm to 450 nm.
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